Dhfr (NM_010049) Mouse Recombinant Protein
Product Specifications
Background
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway (PubMed:25980602) . Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (PubMed:25980602) . Binds its own mRNA.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse dihydrofolate reductase (Dhfr), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
8430436I03Rik; AA607882; AI662710; AW555094
Gene ID
13361
UniProt
P00375, Q544T5
Accession Number mRNA
NM_010049
Chromosomal Location
13 47.64 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
21.6 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Dhfr
Species
Mouse
Protein ID
NP_034179
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/13361
Uniprot URL
https://www.uniprot.org/uniprot/P00375
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_010049
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_034179
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