Dhfr (NM_010049) Mouse Recombinant Protein

Product Specifications

Background

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway (PubMed:25980602) . Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (PubMed:25980602) . Binds its own mRNA.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse dihydrofolate reductase (Dhfr), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

8430436I03Rik; AA607882; AI662710; AW555094

Gene ID

13361

UniProt

P00375, Q544T5

Accession Number mRNA

NM_010049

Chromosomal Location

13 47.64 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

21.6 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.