Cdkn1b (NM_009875) Mouse Recombinant Protein

Product Specifications

Background

Important regulator of cell cycle progression (PubMed:8033213, PubMed:12972555) . Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (By similarity) . Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes (PubMed:8033213) . Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse cyclin-dependent kinase inhibitor 1B (Cdkn1b), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

AA408329; AI843786; Kip1; p27; p27Kip1

Gene ID

12576

UniProt

P46414

Accession Number mRNA

NM_009875

Chromosomal Location

6 65.77 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

22.2 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.