Dtd1 (NM_025314) Mouse Recombinant Protein
Product Specifications
Background
An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA (Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.[UniProtKB/Swiss-Prot Function]
Synonyms
0610006H08Rik; DTD; DUE-B; Hars2
Gene ID
66044
UniProt
Q9DD18
Accession Number mRNA
NM_025314
Chromosomal Location
2 G1
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
23.4 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Dtd1
Species
Mouse
Protein ID
NP_079590
Overview
Purified recombinant protein of Mouse D-tyrosyl-tRNA deacylase 1 (Dtd1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/66044
Uniprot URL
https://www.uniprot.org/uniprot/Q9DD18
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_025314
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_079590
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