Dtd1 (NM_025314) Mouse Recombinant Protein

Product Specifications

Background

An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA (Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse D-tyrosyl-tRNA deacylase 1 (Dtd1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

0610006H08Rik; DTD; DUE-B; Hars2

Gene ID

66044

UniProt

Q9DD18

Accession Number mRNA

NM_025314

Chromosomal Location

2 G1

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

23.4 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.