Gid4 (NM_025757) Mouse Recombinant Protein

Product Specifications

Background

Substrate-recognition subunit of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Binds proteins and peptides with a Pro/N-degron consisting of an unmodified N-terminal Pro followed by a small residue, and has the highest affinity for the peptide Pro-Gly-Leu-Trp. Binds peptides with an N-terminal sequence of the type Pro-[Ala, Gly]-[Leu, Met, Gln, Ser, Tyr]-[Glu, Gly, His, Ser, Val, Trp, Tyr]. Does not bind peptides with an acetylated N-terminal Pro residue.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse GID complex subunit 4, VID24 homolog (Gid4), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

4933439F18Rik

Gene ID

66771

UniProt

Q9CPY6

Accession Number mRNA

NM_025757

Chromosomal Location

11 B2

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

24.9 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.