D2Ertd750e (BC031709) Mouse Recombinant Protein

Product Specifications

Background

Essential component of the mitotic spindle required for faithful chromosome segregation and progression into anaphase. Promotes the metaphase-to-anaphase transition and is required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5) -kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments. Required for kinetochore oscillations and dynamics of microtubule plus-ends during live cell mitosis, possibly by forming a link between spindle microtubule plus-ends and mitotic chromosomes to achieve faithful cell division.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse DNA segment, Chr 2, ERATO Doi 750, expressed (cDNA clone MGC:30961 IMAGE:4021550), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

1700025D04Rik; C15orf23; D2Ertd750e; SKAP; Traf4af1

Gene ID

51944

UniProt

Q9D9Z1

Accession Number mRNA

BC031709

Chromosomal Location

2 E5

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

26.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.