Emg1 (NM_013536) Mouse Recombinant Protein
Product Specifications
Background
S-adenosyl-L-methionine-dependent pseudouridine N (1) -methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3- (3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position. Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes.[UniProtKB/Swiss-Prot Function]
Synonyms
C2f; Grcc2f
Gene ID
14791
UniProt
O35130, Q542P8
Accession Number mRNA
NM_013536
Chromosomal Location
6 59.17 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
27 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Emg1
Species
Mouse
Protein ID
NP_038564
Overview
Purified recombinant protein of Mouse EMG1 N1-specific pseudouridine methyltransferase (Emg1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/14791
Uniprot URL
https://www.uniprot.org/uniprot/O35130
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_013536
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_038564
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