Emg1 (NM_013536) Mouse Recombinant Protein

Product Specifications

Background

S-adenosyl-L-methionine-dependent pseudouridine N (1) -methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3- (3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position. Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse EMG1 N1-specific pseudouridine methyltransferase (Emg1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

C2f; Grcc2f

Gene ID

14791

UniProt

O35130, Q542P8

Accession Number mRNA

NM_013536

Chromosomal Location

6 59.17 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

27 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.