Mettl1 (NM_010792) Mouse Recombinant Protein

Product Specifications

Background

Methyltransferase that mediates the formation of N (7) -methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:29983320) . Catalyzes the formation of N (7) -methylguanine at position 46 (m7G46) in tRNA. Also acts as a methyltransferase for a subset of internal N (7) -methylguanine in mRNAs (PubMed:29983320) . Internal N (7) -methylguanine methylation of mRNAs regulates translation (PubMed:29983320) . Also methylates a specific subset of miRNAs, such as let-7. N (7) -methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA) (By similarity) . Acts as a regulator of embryonic stem cell self-renewal and differentiation (PubMed:29983320) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse methyltransferase like 1 (Mettl1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2810012D02Rik

Gene ID

17299

UniProt

Q9Z120

Accession Number mRNA

NM_010792

Chromosomal Location

10 D3

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

30.6 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.