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Dph5 (NM_027193) Mouse Recombinant Protein

Product Specifications

Background

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse diphthamide biosynthesis 5 (Dph5), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2410012M04Rik; AU045680; C80186

Gene ID

69740

UniProt

Q9CWQ0

Accession Number mRNA

NM_027193

Chromosomal Location

3 G1

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

31.2 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Dph5

Species

Mouse

Protein ID

NP_081469

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/69740

Uniprot URL

https://www.uniprot.org/uniprot/Q9CWQ0

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_027193

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_081469

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