Dph5 (NM_027193) Mouse Recombinant Protein
Product Specifications
Background
S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse diphthamide biosynthesis 5 (Dph5), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
2410012M04Rik; AU045680; C80186
Gene ID
69740
UniProt
Q9CWQ0
Accession Number mRNA
NM_027193
Chromosomal Location
3 G1
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
31.2 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Dph5
Species
Mouse
Protein ID
NP_081469
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/69740
Uniprot URL
https://www.uniprot.org/uniprot/Q9CWQ0
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_027193
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_081469
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