Dohh (NM_133964) Mouse Recombinant Protein

Product Specifications

Background

Catalyzes the hydroxylation of the N (6) - (4-aminobutyl) -L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse deoxyhypusine hydroxylase/monooxygenase (Dohh), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

1110033C18Rik; AI585913; AL022869; Hlrc1

Gene ID

102115

UniProt

Q99LN9

Accession Number mRNA

NM_133964

Chromosomal Location

10 C1

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

32.9 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.