Dohh (NM_133964) Mouse Recombinant Protein
Product Specifications
Background
Catalyzes the hydroxylation of the N (6) - (4-aminobutyl) -L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.[UniProtKB/Swiss-Prot Function]
Synonyms
1110033C18Rik; AI585913; AL022869; Hlrc1
Gene ID
102115
UniProt
Q99LN9
Accession Number mRNA
NM_133964
Chromosomal Location
10 C1
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
32.9 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Dohh
Species
Mouse
Protein ID
NP_598725
Overview
Purified recombinant protein of Mouse deoxyhypusine hydroxylase/monooxygenase (Dohh), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/102115
Uniprot URL
https://www.uniprot.org/uniprot/Q99LN9
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_133964
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_598725
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