Prkra (NM_011871) Mouse Recombinant Protein
Product Specifications
Background
Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1 (By similarity) . Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse protein kinase, interferon inducible double stranded RNA dependent activator (Prkra), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
AV120107; lear; Pact; PRK; RAX
Gene ID
23992
UniProt
Q9WTX2
Accession Number mRNA
NM_011871
Chromosomal Location
2 C3
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
34.4 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Prkra
Species
Mouse
Protein ID
NP_036001
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/23992
Uniprot URL
https://www.uniprot.org/uniprot/Q9WTX2
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_011871
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_036001
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