Prkra (NM_011871) Mouse Recombinant Protein

Product Specifications

Background

Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1 (By similarity) . Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse protein kinase, interferon inducible double stranded RNA dependent activator (Prkra), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

AV120107; lear; Pact; PRK; RAX

Gene ID

23992

UniProt

Q9WTX2

Accession Number mRNA

NM_011871

Chromosomal Location

2 C3

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

34.4 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.