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Auh (NM_016709) Mouse Recombinant Protein

Product Specifications

Background

Catalyzes the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA (By similarity) . Also has itaconyl-CoA hydratase activity by converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate catabolism pathway (By similarity) . The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite (By similarity) . Has very low enoyl-CoA hydratase activity (PubMed:10072761) . Was originally identified as RNA-binding protein that binds in vitro to clustered 5'-AUUUA-3' motifs (PubMed:10072761) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse AU RNA binding protein/enoyl-coenzyme A hydratase (Auh), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

C77140; W91705

Gene ID

11992

UniProt

Q9JLZ3, A0A0R4J023

Accession Number mRNA

NM_016709

Chromosomal Location

13 B1

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

33.8 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Auh

Species

Mouse

Protein ID

NP_057918

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/11992

Uniprot URL

https://www.uniprot.org/uniprot/Q9JLZ3

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_016709

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_057918

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