Auh (NM_016709) Mouse Recombinant Protein
Product Specifications
Background
Catalyzes the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA (By similarity) . Also has itaconyl-CoA hydratase activity by converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate catabolism pathway (By similarity) . The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite (By similarity) . Has very low enoyl-CoA hydratase activity (PubMed:10072761) . Was originally identified as RNA-binding protein that binds in vitro to clustered 5'-AUUUA-3' motifs (PubMed:10072761) .[UniProtKB/Swiss-Prot Function]
Synonyms
C77140; W91705
Gene ID
11992
UniProt
Q9JLZ3, A0A0R4J023
Accession Number mRNA
NM_016709
Chromosomal Location
13 B1
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
33.8 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Auh
Species
Mouse
Protein ID
NP_057918
Overview
Purified recombinant protein of Mouse AU RNA binding protein/enoyl-coenzyme A hydratase (Auh), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/11992
Uniprot URL
https://www.uniprot.org/uniprot/Q9JLZ3
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_016709
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_057918
Curated Selection
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