Npl (NM_028749) Mouse Recombinant Protein

Product Specifications

Background

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse N-acetylneuraminate pyruvate lyase (Npl), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

0610033B02Rik

Gene ID

74091

UniProt

Q9DCJ9

Accession Number mRNA

NM_028749

Chromosomal Location

1 G3

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

35.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.