Nit1 (NM_012049) Mouse Recombinant Protein
Product Specifications
Background
Catalyzes the hydrolysation of the amide bond in N- (4-oxoglutarate) -L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.[UniProtKB/Swiss-Prot Function]
Synonyms
AI255805; ESTM30; W57327
Gene ID
27045
UniProt
Q8VDK1
Accession Number mRNA
NM_012049
Chromosomal Location
1 79.35 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
35.7 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Nit1
Species
Mouse
Protein ID
NP_036179
Overview
Purified recombinant protein of Mouse nitrilase 1 (Nit1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/27045
Uniprot URL
https://www.uniprot.org/uniprot/Q8VDK1
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_012049
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_036179
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