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Akr1d1 (NM_145364) Mouse Recombinant Protein

Product Specifications

Background

Catalyzes the stereospecific NADPH-dependent reduction of the C4-C5 double bond of bile acid intermediates and steroid hormones carrying a delta (4) -3-one structure to yield an A/B cis-ring junction. This cis-configuration is crucial for bile acid biosynthesis and plays important roles in steroid metabolism. Capable of reducing a broad range of delta- (4) -3-ketosteroids from C18 (such as, 17beta-hydroxyestr-4-en-3-one) to C27 (such as, 7alpha-hydroxycholest-4-en-3-one) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse aldo-keto reductase family 1, member D1 (Akr1d1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

MGC25814

Gene ID

208665

UniProt

Q8VCX1

Accession Number mRNA

NM_145364

Chromosomal Location

6 B1

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

37.3 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Akr1d1

Species

Mouse

Protein ID

NP_663339

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/208665

Uniprot URL

https://www.uniprot.org/uniprot/Q8VCX1

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_145364

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_663339

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