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Hmgcl (NM_008254) Mouse Recombinant Protein

Product Specifications

Background

Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S) -3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

AW476067; HL

Gene ID

15356

UniProt

P38060

Accession Number mRNA

NM_008254

Chromosomal Location

4 68.14 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

34.2 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Hmgcl

Species

Mouse

Protein ID

NP_032280

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/15356

Uniprot URL

https://www.uniprot.org/uniprot/P38060

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_008254

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_032280

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