Xrcc4 (NM_028012) Mouse Recombinant Protein

Product Specifications

Background

Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V (D) J recombination. Binds to DNA and to DNA ligase IV (LIG4) . The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse X-ray repair complementing defective repair in Chinese hamster cells 4 (Xrcc4), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2310057B22Rik; AW413319; AW545101

Gene ID

108138

UniProt

Q924T3, A0A0R4J024

Accession Number mRNA

NM_028012

Chromosomal Location

13 C3

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

37.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.