5730593N15Rik (BC025832) Mouse Recombinant Protein
Product Specifications
Background
Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse RIKEN cDNA 5730593N15 gene (cDNA clone MGC:37936 IMAGE:5125723), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
5730593N15Rik
Gene ID
77583
UniProt
Q8R116
Accession Number mRNA
BC025832
Chromosomal Location
11 E2
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
36.8 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
5730593N15Rik
Species
Mouse
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/77583
Uniprot URL
https://www.uniprot.org/uniprot/Q8R116
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/BC025832
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