Mgme1 (NM_028984) Mouse Recombinant Protein

Product Specifications

Background

Metal-dependent single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. Has preference for 5'-3' exonuclease activity but is also capable of endoduclease activity on linear substrates. Necessary for maintenance of proper 7S DNA levels. Probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair (By similarity) . Specifically binds 5-hydroxymethylcytosine (5hmC) -containing DNA in stem cells.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse mitochondrial genome maintenance exonuclease 1 (Mgme1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

8430406I07Rik; AI426476

Gene ID

74528

UniProt

Q9CXC3

Accession Number mRNA

NM_028984

Chromosomal Location

2 G1

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

38.4 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.