Clybl (NM_029556) Mouse Recombinant Protein

Product Specifications

Background

Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism (PubMed:29056341) . Converts citramalyl-CoA into acetyl-CoA and pyruvate in the C5-dicarboxylate catabolism pathway (By similarity) . The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite (PubMed:29056341) . Also acts as a malate synthase in vitro, converting glyoxylate and acetyl-CoA to malate (By similarity) . Also displays malyl-CoA thioesterase activity. Also acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate (By similarity) . Also has very weak citramalate synthase activity in vitro (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse citrate lyase beta like (Clybl), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

0610033J05Rik; 2310014M14Rik; AI256068; Clb

Gene ID

69634

UniProt

Q8R4N0

Accession Number mRNA

NM_029556

Chromosomal Location

14 E5

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

37.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.