Tcea3 (NM_011542) Mouse Recombinant Protein
Product Specifications
Background
Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse transcription elongation factor A (SII), 3 (Tcea3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
S-II; SII-K1
Gene ID
21401
UniProt
P23881
Accession Number mRNA
NM_011542
Chromosomal Location
4 D3
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
38.8 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Tcea3
Species
Mouse
Protein ID
NP_035672
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/21401
Uniprot URL
https://www.uniprot.org/uniprot/P23881
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_011542
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_035672
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