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Tcea3 (NM_011542) Mouse Recombinant Protein

Product Specifications

Background

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse transcription elongation factor A (SII), 3 (Tcea3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

S-II; SII-K1

Gene ID

21401

UniProt

P23881

Accession Number mRNA

NM_011542

Chromosomal Location

4 D3

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

38.8 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Tcea3

Species

Mouse

Protein ID

NP_035672

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/21401

Uniprot URL

https://www.uniprot.org/uniprot/P23881

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_011542

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_035672

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