Vps36 (NM_027338) Mouse Recombinant Protein

Product Specifications

Background

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns (3,4,5) P3.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse vacuolar protein sorting 36 (Vps36), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

1700010A24Rik; 2210415M20Rik; 2810408E15Rik; Eap45

Gene ID

70160

UniProt

Q91XD6, Q3TSR1

Accession Number mRNA

NM_027338

Chromosomal Location

8 A2

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

43.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.