Atg4b (NM_174874) Mouse Recombinant Protein
Product Specifications
Background
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity) .[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse autophagy related 4B, cysteine peptidase (Atg4b), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
2510009N07Rik; Apg4b; Atg4bl; Autl1; AW048066
Gene ID
66615
UniProt
Q8BGE6, A0A0R4J065
Accession Number mRNA
NM_174874
Chromosomal Location
1 D
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
44.9 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Atg4b
Species
Mouse
Protein ID
NP_777363
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/66615
Uniprot URL
https://www.uniprot.org/uniprot/Q8BGE6
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_174874
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_777363
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