Atg4b (NM_174874) Mouse Recombinant Protein

Product Specifications

Background

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse autophagy related 4B, cysteine peptidase (Atg4b), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2510009N07Rik; Apg4b; Atg4bl; Autl1; AW048066

Gene ID

66615

UniProt

Q8BGE6, A0A0R4J065

Accession Number mRNA

NM_174874

Chromosomal Location

1 D

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

44.9 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.