Eif4a2 (NM_013506) Mouse Recombinant Protein
Product Specifications
Background
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.[UniProtKB/Swiss-Prot Function]
Synonyms
4833432N07Rik; BM-010; Ddx2b; eIF-4A-II; Eif4; eIF4A-II
Gene ID
13682
UniProt
P10630, Q52KC1
Accession Number mRNA
NM_013506
Chromosomal Location
16 13.93 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
46.4 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Eif4a2
Species
Mouse
Protein ID
NP_038534
Overview
Purified recombinant protein of Mouse eukaryotic translation initiation factor 4A2 (Eif4a2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/13682
Uniprot URL
https://www.uniprot.org/uniprot/P10630
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_013506
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_038534
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