Trmu (NM_028063) Mouse Recombinant Protein
Product Specifications
Background
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA (Lys), tRNA (Glu) and tRNA (Gln) . Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA (Lys), tRNA (Glu), and tRNA (Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (Trmu), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
1110005N20Rik; 1600025P05Rik; AI314320; Trmt1
Gene ID
72026
UniProt
Q9DAT5
Accession Number mRNA
NM_028063
Chromosomal Location
15 E2
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
47.2 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Trmu
Species
Mouse
Protein ID
NP_082339
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/72026
Uniprot URL
https://www.uniprot.org/uniprot/Q9DAT5
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_028063
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_082339
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