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Chst12 (NM_021528) Mouse Recombinant Protein

Product Specifications

Background

Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse carbohydrate sulfotransferase 12 (Chst12), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

AI595374; C4S-2; C4ST2

Gene ID

59031

UniProt

Q99LL3

Accession Number mRNA

NM_021528

Chromosomal Location

5 G2

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

49.4 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Chst12

Species

Mouse

Protein ID

NP_067503

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/59031

Uniprot URL

https://www.uniprot.org/uniprot/Q99LL3

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_021528

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_067503

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