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Brf2 (NM_025686) Mouse Recombinant Protein

Product Specifications

Background

General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse BRF2, RNA polymerase III transcription initiation factor 50kDa subunit (Brf2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2700059M06Rik; 5730512K07Rik; AU018504; AU022602; BRFU; TFIIIB50

Gene ID

66653

UniProt

Q3UAW9, A0A0R4J0C6

Accession Number mRNA

NM_025686

Chromosomal Location

8 A2

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

47.4 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Brf2

Species

Mouse

Protein ID

NP_079962

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/66653

Uniprot URL

https://www.uniprot.org/uniprot/Q3UAW9

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_025686

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_079962

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