Eif5 (NM_173363) Mouse Recombinant Protein

Product Specifications

Background

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse eukaryotic translation initiation factor 5 (Eif5), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2810011H21Rik; D12Ertd549e

Gene ID

217869

UniProt

P59325, Q3TQR3

Accession Number mRNA

NM_173363

Chromosomal Location

12 61.03 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

49 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.