Eif5 (NM_173363) Mouse Recombinant Protein
Product Specifications
Background
Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]) .[UniProtKB/Swiss-Prot Function]
Synonyms
2810011H21Rik; D12Ertd549e
Gene ID
217869
UniProt
P59325, Q3TQR3
Accession Number mRNA
NM_173363
Chromosomal Location
12 61.03 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
49 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Eif5
Species
Mouse
Protein ID
NP_775539
Overview
Purified recombinant protein of Mouse eukaryotic translation initiation factor 5 (Eif5), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/217869
Uniprot URL
https://www.uniprot.org/uniprot/P59325
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_173363
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_775539
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