Dbt (NM_010022) Mouse Recombinant Protein
Selected Variant
PM46099M5
Size: 20 µg
Product Specifications
Background
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO (2) . It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) . Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse dihydrolipoamide branched chain transacylase E2 (Dbt), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
BCKAD-E2; D3Wsu60e
Gene ID
13171
UniProt
P53395
Accession Number mRNA
NM_010022
Chromosomal Location
3 50.37 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
53.3 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Dbt
Species
Mouse
Protein ID
NP_034152
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/13171
Uniprot URL
https://www.uniprot.org/uniprot/P53395
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_010022
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_034152
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