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Dbt (NM_010022) Mouse Recombinant Protein

Selected Variant

PM46099M5

Size: 20 µg

Product Specifications

Background

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO (2) . It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) . Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse dihydrolipoamide branched chain transacylase E2 (Dbt), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

BCKAD-E2; D3Wsu60e

Gene ID

13171

UniProt

P53395

Accession Number mRNA

NM_010022

Chromosomal Location

3 50.37 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

53.3 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Dbt

Species

Mouse

Protein ID

NP_034152

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/13171

Uniprot URL

https://www.uniprot.org/uniprot/P53395

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_010022

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_034152

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