Rtcb (NM_145422) Mouse Recombinant Protein
Product Specifications
Background
Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs (By similarity) . Essential during post-implantation development of embryos.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse RNA 2', 3'-cyclic phosphate and 5'-OH ligase (Rtcb), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
AI255213; AI463255; D10Wsu52e; FAAP; HSPC117
Gene ID
28088
UniProt
Q99LF4
Accession Number mRNA
NM_145422
Chromosomal Location
10 41.81 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
55.2 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Rtcb
Species
Mouse
Protein ID
NP_663397
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/28088
Uniprot URL
https://www.uniprot.org/uniprot/Q99LF4
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_145422
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_663397
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