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Rtcb (NM_145422) Mouse Recombinant Protein

Product Specifications

Background

Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs (By similarity) . Essential during post-implantation development of embryos.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse RNA 2', 3'-cyclic phosphate and 5'-OH ligase (Rtcb), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

AI255213; AI463255; D10Wsu52e; FAAP; HSPC117

Gene ID

28088

UniProt

Q99LF4

Accession Number mRNA

NM_145422

Chromosomal Location

10 41.81 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

55.2 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Rtcb

Species

Mouse

Protein ID

NP_663397

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/28088

Uniprot URL

https://www.uniprot.org/uniprot/Q99LF4

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_145422

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_663397

Frequently Asked Questions

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