Cyp7b1 (NM_007825) Mouse Recombinant Protein

Product Specifications

Background

Oxysterol 7alpha-hydroxylase that mediates formation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) from 25-hydroxycholesterol (PubMed:9295351) . Plays a key role in cell positioning and movement in lymphoid tissues: 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

AW261589; D3Ertd552e; hct-1

Gene ID

13123

UniProt

Q60991, Q3USU4

Accession Number mRNA

NM_007825

Chromosomal Location

3 4.98 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

58.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.