Nelfa (NM_011914) Mouse Recombinant Protein
Product Specifications
Background
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (By similarity) . The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (By similarity) .[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse negative elongation factor complex member A, Whsc2 (Nelfa), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
Whsc2; Whsc2h
Gene ID
24116
UniProt
Q8BG30
Accession Number mRNA
NM_011914
Chromosomal Location
5 B2
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
57.6 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Nelfa
Species
Mouse
Protein ID
NP_036044
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/24116
Uniprot URL
https://www.uniprot.org/uniprot/Q8BG30
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_011914
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_036044
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