Nelfa (NM_011914) Mouse Recombinant Protein

Product Specifications

Background

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (By similarity) . The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse negative elongation factor complex member A, Whsc2 (Nelfa), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

Whsc2; Whsc2h

Gene ID

24116

UniProt

Q8BG30

Accession Number mRNA

NM_011914

Chromosomal Location

5 B2

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

57.6 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.