Mgrn1 (NM_029657) Mouse Recombinant Protein

Product Specifications

Background

E3 ubiquitin-protein ligase. Mediates TSG101 monoubiquitination at multiple sites. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination (By similarity) . Acts also as a negative regulator of hedgehog signaling (PubMed:29290584) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse mahogunin, ring finger 1 (Mgrn1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2610042J20Rik; md; mKIAA0544; nc

Gene ID

17237

UniProt

Q9D074

Accession Number mRNA

NM_029657

Chromosomal Location

16 2.48 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

58.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.