What is the catalog number for Nae1 (NM_144931) Mouse Recombinant Protein?

The catalog number for Nae1 (NM_144931) Mouse Recombinant Protein is PM45791M5.

What sizes are available for Nae1 (NM_144931) Mouse Recombinant Protein?

Nae1 (NM_144931) Mouse Recombinant Protein is available in the following size(s): 20 µg.

Is Nae1 (NM_144931) Mouse Recombinant Protein in stock?

Nae1 (NM_144931) Mouse Recombinant Protein is currently made to order / not held in immediate stock. Contact us for lead time.

Nae1 (NM_144931) Mouse Recombinant Protein

Product Specifications

Background

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse NEDD8 activating enzyme E1 subunit 1 (Nae1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

59kDa; Appbp1

Gene ID

234664

UniProt

Q8VBW6, Q3UK27

Accession Number mRNA

NM_144931

Chromosomal Location

8 D3

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

60.3 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.