Nae1 (NM_144931) Mouse Recombinant Protein
Product Specifications
Background
Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation (By similarity) .[UniProtKB/Swiss-Prot Function]
Synonyms
59kDa; Appbp1
Gene ID
234664
UniProt
Q8VBW6, Q3UK27
Accession Number mRNA
NM_144931
Chromosomal Location
8 D3
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
60.3 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Nae1
Species
Mouse
Protein ID
NP_659180
Overview
Purified recombinant protein of Mouse NEDD8 activating enzyme E1 subunit 1 (Nae1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/234664
Uniprot URL
https://www.uniprot.org/uniprot/Q8VBW6
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_144931
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_659180
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