Atg9a (BC079884) Mouse Recombinant Protein
Product Specifications
Background
Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H (By similarity) . Required for carbonyl cyanide m-chlorophenylhydrazone (CCCP) -induced ATG8 family proteins lipidation, a key autophagy step.[UniProtKB/Swiss-Prot Function]
Synonyms
Atg9, Atg9l1
Gene ID
245860
UniProt
Q68FE2
Accession Number mRNA
BC079884
Chromosomal Location
1 C4
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
63.1 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Atg9a
Species
Mouse
Overview
Purified recombinant protein of Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/245860
Uniprot URL
https://www.uniprot.org/uniprot/Q68FE2
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/BC079884
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