Atg9a (BC079884) Mouse Recombinant Protein

Product Specifications

Background

Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H (By similarity) . Required for carbonyl cyanide m-chlorophenylhydrazone (CCCP) -induced ATG8 family proteins lipidation, a key autophagy step.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

Atg9, Atg9l1

Gene ID

245860

UniProt

Q68FE2

Accession Number mRNA

BC079884

Chromosomal Location

1 C4

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

63.1 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.