Wee2 (NM_201370) Mouse Recombinant Protein
Product Specifications
Background
Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.[UniProtKB/Swiss-Prot Function]
Synonyms
Gm1065; Wee1B
Gene ID
381759
UniProt
Q66JT0
Accession Number mRNA
NM_201370
Chromosomal Location
6 B1
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
62.2 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Wee2
Species
Mouse
Protein ID
NP_958758
Overview
Purified recombinant protein of Mouse WEE1 homolog 2 (S. pombe) (Wee2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/381759
Uniprot URL
https://www.uniprot.org/uniprot/Q66JT0
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_201370
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_958758
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