Sgpl1 (NM_009163) Mouse Recombinant Protein

Product Specifications

Background

Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine (PubMed:9464245, PubMed:20097939) . Elevates stress-induced ceramide production and apoptosis (PubMed:9464245) . Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts (PubMed:20097939, PubMed:28262793) . Involved in the regulation of pro-inflammatory response and neutrophil trafficking (PubMed:21173151) . Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (PubMed:28521611) . Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse sphingosine phosphate lyase 1 (Sgpl1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

AI428538; D10Xrf456; S1PL; Spl

Gene ID

20397

UniProt

Q8R0X7

Accession Number mRNA

NM_009163

Chromosomal Location

10 32.14 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

63.7 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.