Rec8 (NM_020002) Mouse Recombinant Protein
Product Specifications
Background
Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse REC8 meiotic recombination protein (Rec8), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
Mrec; Rec8L1
Gene ID
56739
UniProt
Q8C5S7
Accession Number mRNA
NM_020002
Chromosomal Location
14 28.19 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
67.4 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Rec8
Species
Mouse
Protein ID
NP_064386
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/56739
Uniprot URL
https://www.uniprot.org/uniprot/Q8C5S7
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_020002
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_064386
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