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Papss1 (BC066055) Mouse Recombinant Protein

Selected Variant

PM45527M5

Size: 20 µg

Product Specifications

Background

Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase) . In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway (PubMed:7493984) . Required for normal biosynthesis of sulfated L-selectin ligands in endothelial cells (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (cDNA clone MGC:91299 IMAGE:6833756), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

AI325286; Asapk; SK1

Gene ID

23971

UniProt

Q60967

Accession Number mRNA

BC066055

Chromosomal Location

3 61.05 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

68.5 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Papss1

Species

Mouse

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/23971

Uniprot URL

https://www.uniprot.org/uniprot/Q60967

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/BC066055

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