Rpn1 (NM_133933) Mouse Recombinant Protein

Product Specifications

Background

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc (3) Man (9) GlcNAc (2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER) . All subunits are required for a maximal enzyme activity.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse ribophorin I (Rpn1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

AU018702; D6Wsu137e; Rpn-1

Gene ID

103963

UniProt

Q91YQ5, Q5RKP4, Q8BMR3

Accession Number mRNA

NM_133933

Chromosomal Location

6 39.13 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

69 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.