Rpn1 (NM_133933) Mouse Recombinant Protein
Product Specifications
Background
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc (3) Man (9) GlcNAc (2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER) . All subunits are required for a maximal enzyme activity.[UniProtKB/Swiss-Prot Function]
Synonyms
AU018702; D6Wsu137e; Rpn-1
Gene ID
103963
UniProt
Q91YQ5, Q5RKP4, Q8BMR3
Accession Number mRNA
NM_133933
Chromosomal Location
6 39.13 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
69 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Rpn1
Species
Mouse
Protein ID
NP_598694
Overview
Purified recombinant protein of Mouse ribophorin I (Rpn1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/103963
Uniprot URL
https://www.uniprot.org/uniprot/Q91YQ5
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_133933
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_598694
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