Pck1 (NM_011044) Mouse Recombinant Protein
Product Specifications
Background
Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate.[UniProtKB/Swiss-Prot Function]
Synonyms
AI265463; Pck-1; PEPCK
Gene ID
18534
UniProt
Q9Z2V4
Accession Number mRNA
NM_011044
Chromosomal Location
2 95.79 cM
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
69.4 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Pck1
Species
Mouse
Protein ID
NP_035174
Overview
Purified recombinant protein of Mouse phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/18534
Uniprot URL
https://www.uniprot.org/uniprot/Q9Z2V4
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_011044
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_035174
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