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Pck1 (NM_011044) Mouse Recombinant Protein

Product Specifications

Background

Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse phosphoenolpyruvate carboxykinase 1, cytosolic (Pck1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

AI265463; Pck-1; PEPCK

Gene ID

18534

UniProt

Q9Z2V4

Accession Number mRNA

NM_011044

Chromosomal Location

2 95.79 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

69.4 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

Pck1

Species

Mouse

Protein ID

NP_035174

Gene ID URL

https://www.ncbi.nlm.nih.gov/gene/18534

Uniprot URL

https://www.uniprot.org/uniprot/Q9Z2V4

Accession Number mRNA URL

https://www.ncbi.nlm.nih.gov/nuccore/NM_011044

Protein ID Link

https://www.ncbi.nlm.nih.gov/nuccore/NP_035174

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