Ndst3 (BC079622) Mouse Recombinant Protein

Product Specifications

Background

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (cDNA clone MGC:90637 IMAGE:30535990), , with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

4921531K01Rik; 4930511P15Rik; N-HSST 3; NDST-3

Gene ID

83398

UniProt

Q9EQH7

Accession Number mRNA

BC079622

Chromosomal Location

3 G1

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

73.8 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.