Lrwd1 (NM_027891) Mouse Recombinant Protein

Product Specifications

Background

Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation (By similarity) . Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse leucine-rich repeats and WD repeat domain containing 1 (Lrwd1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

1200011O22Rik; AU042569; AW548074; Orca

Gene ID

71735

UniProt

Q8BUI3

Accession Number mRNA

NM_027891

Chromosomal Location

5 G2

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

71.6 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.